Thomas Boulin (INMG) has invited Robert Johnston from the Johns Hopkins University to come and give a talk on:
Stochastic gene expression and nuclear architecture in fly eyes and human retinal organoids
A central challenge in developmental neurobiology is to understand how the myriad types of neurons in the human nervous system are generated. Stochastic gene expression mechanisms are crucial to differentiate neuronal subtypes and expand function. During stochastic fate specification, individual neurons randomly choose between different fates, resulting in unique patterns but consistent proportions of cell types among genetically identical organisms. My lab studies the stochastic mechanisms that specify the color-detecting photoreceptors in the fly and human retina. Fruit flies have a well-characterized retina and an abundance of genetic tools that enable molecular analyses of gene regulatory mechanisms. To overcome the challenges associated with human studies, we have developed a human retinal organoid system that recapitulates retinal development and photoreceptor specification. With these systems, we are interrogating how DNA elements, trans factors, and chromatin architecture control random on/off gene expression. Our molecular approaches are complemented by quantitative genetics to determine how natural variation in the genome impacts gene expression and photoreceptor specification. Finally, we conduct behavioral and functional assays to measure differences in color perception when photoreceptor fates are altered. By studying highly divergent organisms from multiple angles, we aim to define the unifying principles underlying stochastic fate specification during nervous system development.
Conference room - Paul Zech library - Thursday 31st May, from 11am